Independent media outlets and an opposition politician said links to Navalny's Smart Voting plan had been blocked.
Leonid Volkov said Putin has not met his international obligations before and any new agreements should take that into account.
The EU issued sanctions on Russian officials connected to opposition leader Alexei Navalny's imprisonment.
Ivan Pavlov, who is defending a former Russian journalist accused of treason, told the press that he was charged with a criminal offense.
Prosecutors in Moscow are now seeking to have Navalny's Anti-Corruption Foundation, labeled as an "extremist" organization, which would fully ban the network's 50 regional headquarters from operating, and put members and supporters at risk of criminal prosecution.
A court found Borovikov guilty of criminal distribution of pornography and sentenced the activist to two and half years in prison.
The Russian opposition leader appeared by video link in a court hearing as he appealed a defamation conviction.
Appealing his conviction in court, the Russian opposition leader spoke by video from prison.
Leonid Volkov, Navalny's chief of staff, told Newsweek the jailed anti-corruption campaigner's case is the "most important" in Russian politics and the biggest challenge to Putin.
A Moscow court restricted the activities of his Russian opposition leader Alexei Navalny's nonprofit organization on Tuesday. It is forbidden from using media or the internet to distribute content or organize rallies, participate in elections or use bank deposits.
In the latest attempt to shut down the operations of Alexei Navalny and his supporters, Russian authorities are asking a Moscow court to label the group as extremists and ban them from spreading information in the media, using banks and organizing public events.
The Russian opposition leader said he is ending his 24-day hunger strike in prison after citing "tremendous progress" from the protest and noting that his health was in serious decline.
The critic of Vladimir Putin had been on hunger strike since March 31 in protest at inadequate medical treatment at his prison.
Into his fourth week of a hunger strike, the opposition leader is languishing in a prison amid growing concerns for his life.
Following the mass protests across Russia on Wednesday on his behalf, political opposition leader Alexei Navalny shared a heartfelt message to his supporters from prison.
The opposition politician is three weeks into a hunger strike and fears are growing for his life, with his personal doctor having warned he could "die any minute."
International media coverage, sanctions and mass protests have failed to puncture the Kremlin's indifference to the health of one of its most prominent critics.
More than 400 arrests have been made at demonstrations across Russia calling for the freedom of opposition leader Alexei Navalny. Navalny's health has reportedly rapidly declined following weeks of a hunger strike while in prison.
Concern is growing for the fragile health of the opposition leader, who has been on hunger strike in prison for three weeks.
The State University of Aerospace Instrumentation in St. Petersburg, Russia, told students on Wednesday that protesting in support of opposition leader Alexei Navalny would get them expelled.
The Russian president said in his annual state-of-the-nation address that his country had been the victim of "illegal" sanctions.
Russian authorities are cracking down on the allies and supporters of political opposition leader Alexei Navalny, as protests are planned in cities across the country.
Demonstrations are scheduled across Russia in support of the jailed Kremlin critic whose health is said to be deteriorating in a prison colony.
Russian opposition leader Alexei Navalny, who is imprisoned and in the midst of a hunger strike, described himself on Tuesday in an Instagram post as "a skeleton" who "staggers around his cell."
The Russian opposition leader has been moved to a new prison hospital known for its brutality against inmates.
The Kremlin critic has been transferred to a prison hospital in a colony his allies say is renowned for its brutality.
The Russian state penitentiary service said Monday that Navalny will be admitted to a hospital in another prison, This comes after the politician's doctor said he could be near death following his third week of a hunger strike while behind bars.
The Russian opposition leader was reportedly transferred to a hospital in another prison after his doctor said he could be near death.
President Putin's most vociferous critic began a hunger strike 20 days ago and doctors have warned that he could suffer cardiac arrest "at any minute."
As Russian opposition leader Alexei Navalny, 44, sits in prison amid an ongoing hunger strike, he used his Instagram account to say that he has been threatened with a straitjacket to be force-fed on Friday.