The women are set to make history when they venture out of the International Space Station to replace a power controller.
The spacewalk is expected to last more than five hours, according to NASA.
This is the first time in over fifty years of space exploration that two women will space-walk at the same time.
An astrophysicist told Newsweek the walk "represents years of dedication, study, hard work, and pushing limits and that is truly inspirational."
The main aim of the spacewalk is to install a special piece of equipment which will enable the space station to accommodate new spacecraft designed by private companies.
Astronauts Drew Feustel and Rocky Arnold will spend more than six hours outside of the ISS Wednesday.
Astronauts took the video while installing a new high-definition camera.
The failed computer controls the major U.S. systems on the International Space Station, including the radiators and cooling loops.
The IMAX documentary "A Beautiful Planet" was filmed by astronauts at the International Space Station.
Several ammonia leaks and minor system failures with the space station's cooling have occurred over the past five years.